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Studies of lysosomal membrane fragility have been carried out in sh and invertebrate species. Exposure to a variety of contaminant ef uents such as sewage sludge, pulp-mill waste, oil spillages and mixed wastes from industry have all been found to increase the fragility of sh hepatocyte lysosomes and molluscan digestive cell lysosomes (Moore, 1985, 1988, 1990; K hler, 1989; K hler et al., 1992; Lowe et al., 1992, 1995a,b; Moore et al., 1996a). In general, the reduction in lysosomal stability is accompanied by enlargement or swelling. Fatty change is also a frequent reaction to xenobiotics in the digestive cells, leading to apparent autophagic uptake of the unsaturated neutral lipid into the often already enlarged lysosomes (Moore, 1988). In order to better understand both the metabolic basis and functional consequences of differences in whole-body protein turnover, procedures have been developed to study the component activities of different proteolytic pathways (Bayne & Hawkins, 1997). Traditionally, it has been thought that requirements for biosynthesis dominate energy expenditure. Nevertheless, among animals generally, a large component of about 30% of the empirical costs of protein deposition cannot be attributed to known synthetic processes, and it has been suggested that costs of protein turnover may contribute to the discrepancy. Bayne and Hawkins (1997) have shown that separate whole-body activities of the four main java ean 13 EAN13 . java · GitHub
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