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The RecA protein has two major properties. First, it coats single-stranded DNA ( g. 12.33) and causes that coated, single-stranded DNA to invade double-stranded DNA ( g. 12.34). By invasion, we mean that the single-stranded DNA attempts to form complementary base pairs with the antiparallel strand of the double-stranded DNA while displacing the other strand of that double helix. A mechanism for this activity, assuming two sites on the enzyme, appears in gure 12.35. RecA continues to move the single-stranded DNA along the double-stranded DNA un-

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Jan 19, 2016 · Convert Tiff file into PDF file using iTextSharp DLL. iTextSharp.text.pdf.PdfWriter writer = iTextSharp.text.pdf.PdfWriter.GetInstance(document, new System.IO.FileStream(destPdf, System.IO.FileMode.Create)); System.Drawing.Bitmap bmp = new System.Drawing.Bitmap(sourceTif); iTextSharp.text.pdf.PdfContentByte cb = writer ...

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For the depletion-mode MOSFET: VP = 5 V IDSS = 7 mA a Are these n- or p-channel devices b Which is the depletion-mode device The enhancement-mode device c For each device, state the conditions for the operation in the active region in terms of the voltages shown for the device and the threshold or pinch-off voltages given above

ampli er stage with: VCC R1 RC RL vi = 15 V C = 047 F = 220 k R2 = 55 k =3k RE = 710 =3k RS = 06 k = Vio sin( t) Vio = 10 mV VCEQ = 761 V

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til a region of homology is found.The second major property of the RecA protein is that, when stimulated by the presence of single-stranded DNA, it causes autocatalysis of another repressor, called LexA, and thus initiates several sequences of reactions. The RecA protein is responsible for filling a postreplicative gap in newly replicated DNA with a strand from the undamaged sister duplex. Gap-filling

_ VGD + G iG + VGS_

Fig 415: A vertical surface grinder [31]

SS _ S iS (a)

(b) Figure 12.34 One property of the RecA protein causes single-

+ G + VGS_ S _

Figure 12.33 Scanning tunneling microscope picture of single-

The transistor is an npn silicon transistor with a transfer characteristic and beta: iC IS evBE /VT = IS eVBEQ +vbe /VT = 100

iS (b)

stranded DNA coated with RecA protein (large arrow). The small arrow indicates uncoated, double-stranded DNA. (In g. 12.35, we show how the very large, coated DNA can invade the very small, uncoated DNA.) ( Science VU-IBMRL/Visuals Unlimited.)

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the combined action of the grinding wheel, the regulating wheel and the blade The grinding pressure is generated dynamically by the difference in the wheel velocity between the regulating and grinding wheels

The device i-v characteristic is plotted in Figure P921 a Determine the no-load large signal gain (vo /vi ) b Sketch the waveform of the output voltage as a function of time c Discuss how the output voltage is distorted compared to the input waveform

+ 25 V 1V + 2V + (b) + 6V 5V + + (c) 2V + (d)

(a) (b)

6 Saturation 5 4 iC (mA) 3 2 1 0

stranded DNA to invade double-stranded DNA and to move along it until a region of complementarity is found. (a) Diagrammatic representation of the invasion of the RecAcoated single-stranded DNA. (b) A more realistic diagram of the same event. (Reproduced, with permission, from the Annual Review of

60 50 AC load line (no load) iB( a) VCE = 6V 40 ic = 2a t = 2 30 t = 0, , 2 20 Q 15 3 t = 2 10 Cutoff 5 1 2 4 6 8 10 12 14 16 18 DC load line VCE(v)

Fig 416: One of the special external centreless grinding operations is the grinding of the balls of ball bearings

25 V + (a)

Biochemistry, Volume 61, 1992 by Annual Reviews, Inc.)

1V

enhancement-mode MOSFET are at potentials of 4 V, 5 V, and 10 V with respect to ground Draw the circuit symbol, with the appropriate voltages at each terminal, if the device is operating a In the ohmic region b In the active region

double-stranded molecule. (a) Axial view of one nucleotide (with two phosphate groups) of single-stranded DNA attached at site I in this cross-sectional diagram of the RecA protein. The protein is about 60% larger than actually shown. (b) Duplex DNA is bound at site II of RecA. (c) RecA protein rotates the bases so that the single-stranded DNA forms a complementary base pair with one strand of the duplex, leaving the other strand of the duplex unpaired (see g. 12.34). (Reprinted with permission from P. Howard-Flanders, et al., Role of RecA protein

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